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Bipotential precursors of B cells and macrophages in murine fetal liver

Making muscle in mammals.

© Uwe Maskos

Tranche d'hippocampe de souris colorée avec deux toxines spécifiques de sous-types de récepteur nicotinique, en rouge (grains), et en vert (corps cellulaires). L'hippocampe est la zone du cerveau qui gère la mémoire spatiale.

Publication : Nucleic acids research

Oligonucleotide hybridizations on glass supports: a novel linker for oligonucleotide synthesis and hybridization properties of oligonucleotides synthesised in situ

Team: Integrative Neurobiology of Cholinergic Systems

Member: Uwe Maskos

Scientific Fields

Diseases

Organisms

Applications

Technique

Published in Nucleic acids research - 11 Apr 1992

Maskos U, Southern EM

Link to Pubmed [PMID] – 1579459

Nucleic Acids Res. 1992 Apr;20(7):1679-84

A novel linker for the synthesis of oligonucleotides on a glass support is described. Oligonucleotides synthesised on the support remain tethered to the support after ammonia treatment and are shown to take part in sequence specific hybridisation reactions. These hybridizations were carried out with oligonucleotides synthesised on ‘ballotini’ solid sphere glass beads and microscope slides. The linker has a hexaethylene glycol spacer, bound to the glass via a glycidoxypropyl silane, terminating in a primary hydroxyl group that serves as starting point for automated or manual oligonucleotide synthesis.

Published on: 11 Apr 1992