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© Research
Publication : Biochemical and biophysical research communications

Nucleotide sequence and tissue distribution of the human mitochondrial aspartate aminotransferase mRNA

Team: Archived: Immunobiology of Dendritic Cells
Member:
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Biochemical and biophysical research communications - 30 Dec 1988

Pol S, Bousquet-Lemercier B, Pave-Preux M, Pawlak A, Nalpas B, Berthelot P, Hanoune J, Barouki R

Link to Pubmed [PMID] – 3207426

Biochem. Biophys. Res. Commun. 1988 Dec;157(3):1309-15

The cDNA of human mitochondrial aspartate aminotransferase (E.C.2.6.1.1.) was isolated from a human liver cDNA library using a rat mitochondrial aspartate aminotransferase cDNA as probe. The sequence of this cDNA gives a predicted aminoacid sequence for the human presequence and for the human mature protein exhibiting respectively 93% and 95% homology with rat sequences. A Northern blot of total RNA, isolated from various human tissues and hybridized with this cDNA, revealed a single 2.4 Kb RNA band. Mitochondrial aspartate aminotransferase RNA was clearly detected in human kidney, placenta, stomach and spleen as well as in both fetal and adult liver.