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© Mélanie Falord, Tarek Msadek, Jean-Marc Panaud
Staphylococcus aureus "golden staph" in scanning electron microscopy.
Publication : Applied and environmental microbiology

New vector for efficient allelic replacement in naturally nontransformable, low-GC-content, gram-positive bacteria

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Applied and environmental microbiology - 01 Nov 2004

Arnaud M, Chastanet A, Débarbouillé M

Link to Pubmed [PMID] – 15528558

Appl. Environ. Microbiol. 2004 Nov;70(11):6887-91

A shuttle vector designated pMAD was constructed for quickly generating gene inactivation mutants in naturally nontransformable gram-positive bacteria. This vector allows, on X-Gal (5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside) plates, a quick colorimetric blue-white discrimination of bacteria which have lost the plasmid, greatly facilitating clone identification during mutagenesis. The plasmid was used in Staphylococcus aureus, Listeria monocytogenes, and Bacillus cereus to efficiently construct mutants with or without an associated antibiotic resistance gene.

http://www.ncbi.nlm.nih.gov/pubmed/15528558