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© Emeline Camand
Marquage par immunofluorescence d'astrocytes tumoraux ou astrocytomes (lignée cellulaire humaine U373), montrant en rouge, APC et en vert, la tubuline des microtubules. APC est un supresseur de tumeur qui est impliqué dans la polarisation des astrocytes normaux. La localisation d'APC est altérée dans des lignées de gliomes. Pour essayer de corriger, les dérèglements observés lors de la migration des cellules d'astrocytes tumuraux ou gliomes on cherche à connaitre les mécanismes moléculaires fondamentaux qui controlent la polarisation et la migration cellulaire.
Publication : Comptes rendus de l'Académie des sciences. Série III, Sciences de la vie

[Neutralising properties for HIV virus of hybrid protein MalE-CD4 expressed in E. coli and purified in 1 step]

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Comptes rendus de l'Académie des sciences. Série III, Sciences de la vie - 01 Jan 1989

Clément JM, Szmelcman S, Jehanno M, Martineau P, Schwartz O, Hofnung M

Link to Pubmed [PMID] – 2541875

C. R. Acad. Sci. III, Sci. Vie 1989;308(14):401-6

Genetic fusions allowing the expression in E. coli of hybrid proteins between a bacterial periplasmic maltose binding protein (MalE) and the CD4 molecule (the receptor of the HIV virus) have been constructed. One of them has kept most of the properties of each constituent: it is exported, can be purified in one step on an affinity column, interacts with anti-MalE and anti-CD4 antibodies, binds HIV gp 120 protein and inactivates HIV virus in an in vitro test.

http://www.ncbi.nlm.nih.gov/pubmed/2541875