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© Sandrine Etienne-Manneville
Photo prise à l'avant (dans la protrusion) d'astrocytes primaires de rat en migration. Marquage par immunofluorescence montrant en rouge, p150 Glued, une protéine associée aux extrémités 'plus' des microtubules et en vert la tubuline des microtubules. La photographie montre l'accumulation de p150 Glued à l'avant des cellules en migration, où la protéine pourrait participer à l'ancrage des microtubules à la membrane plasmique. Pour essayer de corriger, les dérèglements observés lors de la migration des cellules d'astrocytes tumuraux ou gliomes on cherche à connaitre les mécanismes moléculaires fondamentaux qui controlent la polarisation et la migration cellulaires.
Publication : BMC genomics

Multiple displacement amplification for complex mixtures of DNA fragments

Team: Biochemistry of Macromolecular Interactions
Member: Undine Mechold
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in BMC genomics - 15 Sep 2008

Shoaib M, Baconnais S, Mechold U, Le Cam E, Lipinski M, Ogryzko V

Link to Pubmed [PMID] – 18793430

BMC Genomics 2008;9:415

A fundamental requirement for genomic studies is the availability of genetic material of good quality and quantity. The desired quantity and quality are often hard to obtain when target DNA is composed of complex mixtures of relatively short DNA fragments. Here, we sought to develop a method to representatively amplify such complex mixtures by converting them to long linear and circular concatamers, from minute amounts of starting material, followed by phi29-based multiple displacement amplification.