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© Research
Publication : The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society

Microdissection-derived murine mcb probes from somatic cell hybrids

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society - 01 Jun 2005

Trifonov V, Karst C, Claussen U, Mrasek K, Michel S, Avner P, Liehr T

Link to Pubmed [PMID] – 15928329

J. Histochem. Cytochem. 2005 Jun;53(6):791-2

The multicolor-banding (mcb) technique is a fluorescence in situ hybridization (FISH)-banding approach, which is based on region-specific microdissection libraries producing changing fluorescence intensity ratios along the chromosomes. The latter are used to assign different pseudocolors to specific chromosomal regions. Here we present the first three available mcb-probe sets for the Mus musculus chromosomes 3, 6, and 18. In the present work, the creation of the microdissection libraries was done for the first time on mouse/human somatic cell hybrids. During creation of the mcb-probes, the latter enabled an unambiguous identification of the, otherwise in GTG-banding, hardly distinguishable murine chromosomes.

http://www.ncbi.nlm.nih.gov/pubmed/15928329