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© Research
Publication : Saudi journal of biological sciences

MALDI-TOF-MS and 16S rRNA characterization of lead tolerant metallophile bacteria isolated from saffron soils of Kashmir for their sequestration potential.

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Saudi journal of biological sciences - 01 Aug 2020

Uqab B, Nazir R, Ahmad Ganai B, Rahi P, Rehman S, Farooq S, Dar R, Parray JA, Fahad Al-Arjani Al-Arjani AB, Tabassum B, Fathi Abd Allah E,

Link to Pubmed [PMID] – 32714029

Link to DOI – 10.1016/j.sjbs.2020.04.021

Saudi J Biol Sci 2020 Aug; 27(8): 2047-2053

Toxic metal contamination in soils due industrialization is nowadays a concern to the scientists worldwide. The current study deals with the evaluation of response and tolerance by isolated metallophilic bacteria in different lead concentrations (100 ppm to 1000 ppm). By taking optical densities of the isolates, the minimum inhibitory concentration (MIC) of Pb2+ were determined.16S rRNA and MALDI-TOF MS were used for the identification of the bacteria. Total of 37 isolates were observed, among them 04 (Staphylococcus equorum, Staphylococcus warneri, Bacillus safensis and Bacillus thuringiensis), isolated were detected having efficacy of Pb2+tolerance and sequestration at varying MIC. Furthermore, B. thuringiensis was observed to have highest (900 ppm) tolerance for lead and lowest (500 ppm) for Staphylococcus warneri. Moreover, the highest (65.3%) sequestration potential has been observed for B. thuringiensis and least (52.8%) for S. warneri. The tolerance and sequestration potential properties of these isolated species can be utilised to exterminate heavy metals and reduce their toxicity from the contaminated environment.

https://pubmed.ncbi.nlm.nih.gov/32714029