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© Research
Publication : Carbohydrate research

Low molecular weight chitosans–preparation by depolymerization with Aspergillus niger pectinase, and characterization

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Carbohydrate research - 16 Jun 2003

Kittur FS, Vishu Kumar AB, Tharanathan RN

Link to Pubmed [PMID] – 12791281

Carbohydr. Res. 2003 Jun;338(12):1283-90

The viscosity of a chitosan solution was rapidly lowered in the presence of pectinase from Aspergillus niger at pH 3.0 and 37 degrees C. The low molecular weight chitosans (LMWC) had a molecular weight in the range 20,000-5000 Da. Circular dichroism spectra showed a decrease in the segment of acetylated glucosamine units, whereas X-ray diffraction and CP-MAS 13C NMR indicated higher crystallinity and polymorphism in LMWC. The latter on thermal drying resulted in structural alterations, and yielded an insoluble product. FT-IR and X-ray diffraction showed no evidence of either Schiff’s base linkage or any annealed polymorph. CP-MAS 13C NMR showed marked changes in the chain conformations of LMWC, which are believed to be responsible for its loss of solubility and functionality.

http://www.ncbi.nlm.nih.gov/pubmed/12791281