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© Research
Publication : Journal of bacteriology

IS1397 is active for transposition into the chromosome of Escherichia coli K-12 and inserts specifically into palindromic units of bacterial interspersed mosaic elements

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Journal of bacteriology - 01 Nov 1999

Clément JM, Wilde C, Bachellier S, Lambert P, Hofnung M

Link to Pubmed [PMID] – 10559158

J. Bacteriol. 1999 Nov;181(22):6929-36

We demonstrate that IS1397, a putative mobile genetic element discovered in natural isolates of Escherichia coli, is active for transposition into the chromosome of E. coli K-12 and inserts specifically into palindromic units, also called repetitive extragenic palindromes, the basic element of bacterial interspersed mosaic elements (BIMEs), which are found in intergenic regions of enterobacteria closely related to E. coli and Salmonella. We could not detect transposition onto a plasmid carrying BIMEs. This unprecedented specificity of insertion into a well-characterized chromosomal intergenic repeated element and its evolutionary implications are discussed.

http://www.ncbi.nlm.nih.gov/pubmed/10559158