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Publication : Journal of Electron Spectroscopy and Related Phenomena

Irradiation damage to frog inner ear during synchrotron radiation tomographic investigation

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Journal of Electron Spectroscopy and Related Phenomena - 01 Mar 2009

Renaud Boistel, Nicolas Pollet, Jean-Yves Tinevez, Peter Cloetens, Michel Schlenker

Journal of Electron Spectroscopy and Related Phenomena, Volume 170, Issues 1–3, 2009, Pages 37-41

Unexpectedly severe radiation damage, showing up through deformation of the saccule, was encountered during a synchrotron radiation high-resolution (700 nm pixel size) tomographic observation of an inner ear, fixed in a formaldehyde solution, of the frog Rana esculenta. The visible displacement of the edge of the otoconia-filled part of the saccule amounted to about 100 μm after an irradiation with 20.5 keV X-ray photons corresponding to a dose of 1.5 kGy for the protein matrix. The close-knit coexistence of organic and mineral components in the biological tissue may be linked to the dramatic increase of radiation dosage sensitivity.