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© Institut Pasteur
Phlébotome. Petit diptère de la famille des psychodidés (dans l'Ancien Monde). La femelle hématophage peut être vectrice entre autres de leishmaniose qu'elle transmet par piqûre à un vertébré, chien (hôte principal réservoir), homme ou encore rat (hôtes occasionnels).
Publication : Journal of molecular biology

Innovative Toolbox for the Quantification of Drosophila C Virus, Drosophila A Virus, and Nora Virus.

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Journal of molecular biology - 30 Mar 2022

Nigg JC, Mongelli V, Blanc H, Saleh MC,

Link to Pubmed [PMID] – 34678301

Link to DOI – 10.1016/j.jmb.2021.167308

J Mol Biol 2022 03; 434(6): 167308

Quantification of viral replication underlies investigations into host-virus interactions. In Drosophila melanogaster, persistent infections with Drosophila C virus, Drosophila A virus, and Nora virus are commonly observed in nature and in laboratory fly stocks. However, traditional endpoint dilution assays to quantify infectious titers are not compatible with persistently infecting isolates of these viruses that do not cause cytopathic effects in cell culture. Here we present a novel assay based on immunological detection of Drosophila C virus infection that allows quantification of infectious titers for a wider range of Drosophila C virus isolates. We also describe strand specific RT-qPCR assays for quantification of viral negative strand RNA produced during Drosophila C virus, Drosophila A virus, and Nora virus infection. Finally, we demonstrate the utility of these assays for quantification of viral replication during oral infections and persistent infections with each virus.

https://pubmed.ncbi.nlm.nih.gov/34678301