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© Research
Publication : Archives of virology

Infection of barley protoplasts with rice hoja blanca tenuivirus. Brief report

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Archives of virology - 01 Jan 1999

Nguyen M, Kormelink R, Goldbach R, Haenni AL

Link to Pubmed [PMID] – 10603179

Arch. Virol. 1999;144(11):2247-52

A barley protoplast system has been established that supports replication of Rice hoja blanca tenuivirus (RHBV). Following polyethylene glycol-mediated RHBV inoculation of barley protoplasts, newly synthesized viral RNAs and proteins could be detected. Time course analyses revealed de novo synthesis of genome length viral RNA4, as well as subgenomic-sized RNA4 molecules of both polarities. Two proteins, N and NS4, encoded by viral complementary RNA3 and viral RNA4 respectively, were detected by Western immunoblot analysis. The barley protoplast system thus constitutes a promising tool for in vivo studies of the sequential steps involved in the multiplication cycle of RHBV.