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© Research
Publication : FEBS letters

Sequence analysis of a monoclonal antibody specific for the preS2 region of hepatitis B surface antigen, and the cloning, expression and characterisation of its single-chain Fv construction

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in FEBS letters - 28 Dec 1998

Passafiume M, Vulliez-le Normand B, Riottot MM, Bentley GA

Link to Pubmed [PMID] – 9891981

FEBS Lett. 1998 Dec;441(3):407-12

The nucleotide sequence of the monoclonal antibody F124, specific for the preS2 region of the surface antigen of hepatitis B virus, has been determined and an single-chain Fv fragment (scFv) recombinant construction has been cloned and expressed into the periplasmic region of Escherichia coli. The recombinant antibody fragment contains a (Gly4Ser)3 linker connecting the C-terminus of the heavy chain variable region (V(L)) domain to the N-terminus of the light chain variable region (V(L)) domain. A 23-residue peptide segment, containing a c-myc marker for immunochemical detection of the scFv and hexahistidine tag to facilitate its purification, was added C-terminal to the V(L) domain. The scFv mimics the antibody in binding to the native antigen in the form of recombinant hepatitis B surface antigen (HBsAg) particles as well as to peptide fragments carrying the viral epitope.

http://www.ncbi.nlm.nih.gov/pubmed/9891981