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© Aline Bonnet, Institut Pasteur
Coupe transversale d’embryon de caille transgénique mbGFP à 18somites, au niveau du futur bourgeon de membre antérieur avec un marquage noyaux (bleu), GFP (vert) et actine (rouge) / Transversal section of a mbGFP transgenic quail embryo at 18-somite stage, at forelimb level, with nuclei (blue), GFP (green) and actin (red) labelling
Publication : Developmental dynamics : an official publication of the American Association of Anatomists

In ovo electroporation of avian somites

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Developmental dynamics : an official publication of the American Association of Anatomists - 01 Mar 2004

Scaal M, Gros J, Lesbros C, Marcelle C

Link to Pubmed [PMID] – 14991719

Dev. Dyn. 2004 Mar;229(3):643-50

In ovo electroporation is a well-established method of gene transfer into neural and mesenchymal tissue in chick embryos. Electroporation of somites, however, has been hampered by low efficiency due to technical difficulties. Here, we present a powerful technique to electroporate avian somites and subpopulations of somitic cells at high efficiency in ovo. We demonstrate specific targeting of distinct somitic compartments and their derivatives using single or combinations of plasmid expression vectors. This technique opens new perspectives to investigate the morphologic and genetic basis of somite development.

http://www.ncbi.nlm.nih.gov/pubmed/14991719