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© Research
Publication : Journal of microscopy

Image registration and distortion correction in ion microscopy

Team: Biological Image Analysis
Member: Jean-Christophe Olivo-Marin
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Journal of microscopy - 01 Dec 1991

Olivo JC, Kahn E, Halpern S, Fragu P

Link to Pubmed [PMID] – 1795385

J Microsc 1991 Dec;164(Pt 3):263-72

We present a method whereby the geometric registration of a series of ion microscopic images is performed by applying a two-step procedure. After applying a global linear transformation that corrects for geometric differences, a non-linear elastic transform is used in order to match local properties and structures of the images. Transformation parameters are computed on the basis of shape-specific points in the images. Distortion correction is achieved by relating ion images to the optical image of the same field and by using the two-step algorithm to register the images. This methodology is evaluated on synthetic misaligned objects and on thyroid tissue images.