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© Research
Publication : PloS one

Host-Associated Metagenomics: A Guide to Generating Infectious RNA Viromes

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in PloS one - 02 Oct 2015

Temmam S, Monteil-Bouchard S, Robert C, Pascalis H, Michelle C, Jardot P, Charrel R, Raoult D, Desnues C

Link to Pubmed [PMID] – 26431175

PLoS ONE 2015;10(10):e0139810

Metagenomic analyses have been widely used in the last decade to describe viral communities in various environments or to identify the etiology of human, animal, and plant pathologies. Here, we present a simple and standardized protocol that allows for the purification and sequencing of RNA viromes from complex biological samples with an important reduction of host DNA and RNA contaminants, while preserving the infectivity of viral particles.

https://www.ncbi.nlm.nih.gov/pubmed/26431175