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© Research
Publication : Genes

Golgi Reassembly and Stacking Protein (GRASP) Participates in Vesicle-Mediated RNA Export in Cryptococcus Neoformans

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Genes - 08 Aug 2018

Peres da Silva R, Martins ST, Rizzo J, Dos Reis FCG, Joffe LS, Vainstein M, Kmetzsch L, Oliveira DL, Puccia R, Goldenberg S, Rodrigues ML, Alves LR

Link to Pubmed [PMID] – 30096850

Genes (Basel) 2018 Aug;9(8)

Golgi reassembly and stacking protein (GRASP) is required for polysaccharide secretion and virulence in . In fungal species, extracellular vesicles (EVs) participate in the export of polysaccharides, proteins and RNA. In the present work, we investigated if EV-mediated RNA export is functionally connected with GRASP in using a Δ mutant. Since GRASP-mediated unconventional secretion involves autophagosome formation in yeast, we included the Δ mutant with defective autophagic mechanisms in our analysis. All fungal strains exported EVs but deletion of or profoundly affected vesicular dimensions. The mRNA content of the Δ EVs differed substantially from that of the other two strains. The transcripts associated to the endoplasmic reticulum were highly abundant transcripts in Δ EVs. Among non-coding RNAs (ncRNAs), tRNA fragments were the most abundant in both mutant EVs but Δ EVs alone concentrated 22 exclusive sequences. In general, our results showed that the EV RNA content from Δ and WT were more related than the RNA content of Δ, suggesting that GRASP, but not the autophagy regulator Atg7, is involved in the EV export of RNA. This is a previously unknown function for a key regulator of unconventional secretion in eukaryotic cells.

https://www.ncbi.nlm.nih.gov/pubmed/30096850