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© Research
Publication : Methods Mol Biol

Generation and Analysis of Chromosomal Contact Maps of Yeast Species

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Methods Mol Biol - 01 Jan 2016

Cournac A, Marbouty M, Mozziconacci J, Koszul R

Link to Pubmed [PMID] – 26483025

Methods Mol Biol. 2016;1361:227-45

Genome-wide derivatives of the chromosome conformation capture (3C) technique are now well-established approaches to study the multiscale average organization of chromosomes from bacteria to mammals. However, the experimental parameters of the protocol have to be optimized for different species, and the downstream experimental products (i.e., pair-end sequences) are influenced by these parameters. Here, we describe a complete pipeline to generate 3C-seq libraries and compute chromosomal contact maps of yeast species.

http://link.springer.com/protocol/10.1007%2F978-1-4939-3079-1_13