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© Research
Publication : FEMS microbiology letters

Evaluation of 16S rDNA- and gyrB-DGGE for typing members of the genus Aeromonas

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in FEMS microbiology letters - 01 May 2005

Tacão M, Moura A, Alves A, Henriques I, Saavedra MJ, Correia A

Link to Pubmed [PMID] – 15869956

FEMS Microbiol. Lett. 2005 May;246(1):11-8

The DNA sequence of the gyrB gene is a suitable phylogenetic marker for bacterial systematics. In this study, the diversity of Aeromonas spp. present in environmental samples was assessed by a PCR combined with DGGE approach. PCR primers targeting the gyrB gene of aeromonads were designed and the resulting amplicons were analyzed by DGGE. The gyrB-DGGE analysis was evaluated with Aeromonas isolates and reference strains allowing discrimination of the majority of strains. The gyrB-DGGE analysis is a powerful tool to monitor the presence and evaluate the diversity of aeromonads in complex samples, as is the case of water from a wastewater treatment plant.

http://www.ncbi.nlm.nih.gov/pubmed/15869956