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© Pierre Lafaye
Astrocytes marqués par des anticorps VHH anti-GFAP. Des anticorps d'alpagas dirigés contre une protéine spécifique des astrocytes, la GFAP (Glial Fibrillary Acidic Protein), ont été obtenus à partir de camélidés immunisés. La partie VHH (partie de l'anticorps qui reconnaît l'antigène) a été exprimée sous forme recombinante chez Escherichia coli.
Publication : Annales de l'Institut Pasteur. Immunologie

Enzyme-linked immunosorbent assay for measurement of penicilloyl groups

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Annales de l'Institut Pasteur. Immunologie - 01 May 1985

Lapresle C, Lafaye P

Link to Pubmed [PMID] – 4037729

Ann. Inst. Pasteur Immunol. 1985 May-Jun;136C(3):375-82

An enzyme-linked immunosorbent assay for measuring penicillin groups is described. Alkaline phosphatase conjugated to penicilloyl residue was reacted with purified anti-penicilloyl antibodies coated upon polystyrene plates. The inhibition of this reaction allowed a specific determination of penicilloyl residues at the picomole level. This assay was applied to the study of the penicilloyl groups linked to human albumin and to the measurement of some of these groups after enzymatic degradation or physical modifications of the albumin molecule.

http://www.ncbi.nlm.nih.gov/pubmed/4037729