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© Research
Publication : Methods in molecular biology (Clifton, N.J.)

Enrichment of unstable non-coding RNAs and their genome-wide identification

Member: Alain Jacquier
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Methods in molecular biology (Clifton, N.J.) - 01 Jan 2011

Neil H, Jacquier A

Link to Pubmed [PMID] – 21863483

Methods Mol. Biol. 2011;759:87-106

Cryptic unstable transcripts (CUTs) have been recently described as a major class of non-coding RNAs. These transcripts are, however, extremely unstable in normal cells and their analyzes pose specific technical problems. In this chapter, after a brief introduction discussing general aspects associated with the analysis of non-coding RNAs, we provide details of methods to enrich, map, and quantify this unconventional class of transcripts.