Link to Pubmed [PMID] – 9461480
Nucleic Acids Res. 1998 Feb; 26(4): 1130-1
A new method for specific reamplification of DDRT-PCR products is presented. After transient ligation of the primary DDRT-PCR fragments into a T-vector, the cDNAs of interest were reamplified by hemi-nested PCR and thermally asymmetric cycles. In contrast to the originally described protocol, this method of reamplification is specific, sensitive, reproducibly gives a high yield of DNA and allows direct sequencing of the reamplified product without purification or cloning.
https://pubmed.ncbi.nlm.nih.gov/9461480