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© Research
Publication : Nucleic acids research

Efficient reamplification of differential display products by transient ligation and thermal asymmetric PCR.

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Nucleic acids research - 15 Feb 1998

Bonnet S, Prévot G, Bourgouin C,

Link to Pubmed [PMID] – 9461480

Nucleic Acids Res. 1998 Feb; 26(4): 1130-1

A new method for specific reamplification of DDRT-PCR products is presented. After transient ligation of the primary DDRT-PCR fragments into a T-vector, the cDNAs of interest were reamplified by hemi-nested PCR and thermally asymmetric cycles. In contrast to the originally described protocol, this method of reamplification is specific, sensitive, reproducibly gives a high yield of DNA and allows direct sequencing of the reamplified product without purification or cloning.

https://pubmed.ncbi.nlm.nih.gov/9461480