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© Research
Publication : Nucleic Acids Research

Efficient reamplification of differential display products by transient ligation and thermal asymmetric PCR

Team: Group: Catherine Bourgouin
Team: Ecology and Emergence of Arthropod-borne Pathogens
Member: Catherine Bourgouin
Member: Sarah Bonnet
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Nucleic Acids Research - 01 Feb 1998

S Bonnet, Ghislaine Prevot, C Bourgouin

Link to Pubmed [PMID] – 9461480

Link to HAL – univ-guyane-03289316

Link to DOI – 10.1093/nar/26.4.1130

Nucleic Acids Research, 1998, 26 (4), pp.1130-1131. ⟨10.1093/nar/26.4.1130⟩

A new method for specific reamplification of DDRT-PCR products is presented. After transient ligation of the primary DDRT-PCR fragments into a T-vector, the cDNAs of interest were reamplified by hemi-nested PCR and thermally asymmetric cycles. In contrast to the originally described protocol, this method of reamplification is specific, sensitive, reproducibly gives a high yield of DNA and allows direct sequencing of the reamplified product without purification or cloning.