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© Research
Publication : Molecular cell

Dynamic Processing of Displacement Loops during Recombinational DNA Repair

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Molecular cell - 05 Feb 2019

Piazza A, Shah SS, Wright WD, Gore SK, Koszul R, Heyer WD

Link to Pubmed [PMID] – 30737186

Mol. Cell 2019 03;73(6):1255-1266.e4

Displacement loops (D-loops) are pivotal intermediates of homologous recombination (HR), a universal DNA double strand break (DSB) repair pathway. We developed a versatile assay for the physical detection of D-loops in vivo, which enabled studying the kinetics of their formation and defining the activities controlling their metabolism. Nascent D-loops are detected within 2 h of DSB formation and extended in a delayed fashion in a genetic system designed to preclude downstream repair steps. The majority of nascent D-loops are disrupted by two pathways: one supported by the Srs2 helicase and the other by the Mph1 helicase and the Sgs1-Top3-Rmi1 helicase-topoisomerase complex. Both pathways operate without significant overlap and are delineated by the Rad54 paralog Rdh54 in an ATPase-independent fashion. This study uncovers a layer of quality control of HR relying on nascent D-loop dynamics.

https://www.ncbi.nlm.nih.gov/pubmed/30737186