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© Research
Publication : Current microbiology

Distribution of the insertion element IS240 among Bacillus thuringiensis strains.

Member: Marie-Laure Rosso
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Current microbiology - 01 Jun 1997

Rosso ML, Delécluse A,

Link to Pubmed [PMID] – 9142741

Curr. Microbiol. 1997 Jun; 34(6): 348-53

The presence of IS240 was investigated in 69 Bacillus thuringiensis (Bt) strains including strains from serotype H1 to H45 and additional strains with known Dipteran larvae toxicity. Restriction digests of total DNA and PCR products obtained with a single 16-bases primer corresponding to the IS240 inverted repeated sequence were hybridized with the IS240A element. The results indicate that 67% of the Bt strains tested, including all known mosquitocidal strains, possess at least one IS240-related element. PCR experiments indicate that IS240 represents a family of insertion sequences with several variants.