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© Research
Publication : Science (New York, N.Y.)

Direct isolation of satellite cells for skeletal muscle regeneration

Member: Ana Cumano
Member: Didier Montarras
Member: Margaret Buckingham
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Science (New York, N.Y.) - 01 Sep 2005

Montarras D, Morgan J, Collins C, Relaix F, Zaffran S, Cumano A, Partridge T, Buckingham M

Link to Pubmed [PMID] – 16141372

Science 2005 Sep;309(5743):2064-7

Muscle satellite cells contribute to muscle regeneration. We have used a Pax3(GFP/+) mouse line to directly isolate (Pax3)(green fluorescent protein)-expressing muscle satellite cells, by flow cytometry from adult skeletal muscles, as a homogeneous population of small, nongranular, Pax7+, CD34+, CD45-, Sca1- cells. The flow cytometry parameters thus established enabled us to isolate satellite cells from wild-type muscles. Such cells, grafted into muscles of mdx nu/nu mice, contributed both to fiber repair and to the muscle satellite cell compartment. Expansion of these cells in culture before engraftment reduced their regenerative capacity.