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© A. Alanio, E. Perret
Prolifération de Cryptococcus neoformans dans des macrophages murins.
Publication : BMC infectious diseases

Direct genotyping of Toxoplasma gondii from amniotic fluids based on B1 gene polymorphism using minisequencing analysis

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in BMC infectious diseases - 19 Nov 2013

Costa JM, Alanio A, Moukoury S, Clairet V, Debruyne M, Poveda JD, Bretagne S

Link to Pubmed [PMID] – 24252188

BMC Infect. Dis. 2013;13:552

Because some Toxoplasma gondii genotypes may be more virulent in pregnant women, discriminating between them appears valuable. Currently, the main genotyping method is based on single copy microsatellite markers, which limit direct genotyping from amniotic fluids (AFs) to samples with a high parasitic load. We investigated whether the multicopy gene B1 could type the parasite with a higher sensitivity. To estimate the amplifiable DNA present in AFs, we first compared three different PCR assays used for Toxoplasma infection diagnosis: the P30-PCR, targeting the single copy gene P30; the B1-PCR, targeting the repeated B1 gene; and RE-PCR, targeting the repeated element.

http://www.ncbi.nlm.nih.gov/pubmed/24252188