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© Michel Huerre
Coupe histologique de foie de patient atteint d'une hépatite C chronique active avec infiltrat (opacité) folliculaire (lymphocytes) (Grossissement X 400). L'hépatite C chronique peut être à l'origine de cirrhose et cancer du foie
Publication : Journal of virological methods

Development of a RT-nested PCR test detecting pigeon Paramyxovirus-1 directly from organs of infected animals

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Journal of virological methods - 01 Dec 2002

Barbezange C, Jestin V

Link to Pubmed [PMID] – 12393150

J. Virol. Methods 2002 Dec;106(2):197-207

A RT-nested PCR that amplifies part of the conserved nucleoprotein gene of avian Paramyxovirus type 1 is described. The technique allowed the detection of pigeon Paramyxovirus type 1 (pPMV-1) virus directly from a wide range of infected chicken and pigeon organs, and should be able to detect typical Newcastle disease viruses too. Compared with the reference method, the developed RT-nested PCR was found more sensitive, as it was able to detect virus genome in infected pigeon organs at late stage of infection, when virus isolation failed. Such a molecular technique represents an alternative method of diagnosis for research purposes on pPMV-1 variants, for example to study pathogenesis aspects of the infection or to assess the efficacy of vaccines.

http://www.ncbi.nlm.nih.gov/pubmed/12393150