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© Sophie Novault, Institut Pasteur
Publication : STAR Protoc

Deep phenotyping characterization of human unconventional CD8+NKG2A/C+ T cells among T and NK cells by spectral flow cytometry.

Team: Flow Cytometry Platform
Member: Sandrine Schmutz
Member: Sophie Novault
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in STAR Protoc - 15 Dec 2023

Orta-Resendiz A, Petitdemange C, Schmutz S, Jacquelin B, Novault S, Huot N, Müller-Trutwin M.

Link to Pubmed [PMID] – 38032799

Link to DOI – 10.1016/j.xpro.2023.102734

STAR Protoc. 2023 Nov 29;4(4):102734

Here, we present a protocol for setting three spectral flow cytometry panels for the characterization of human unconventional CD8+NKG2A/C+ T cells as well as other T and natural killer cell subsets. We describe steps for standardizing, preparing, and staining the cells, the experimental setup, and the final data analysis. This protocol should be advantageous in various settings including immunophenotyping of limited samples, immune function evaluation/monitoring, as well as research in oncology, autoimmune, and infectious diseases.