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© Christine Schmitt, Meriem El Ghachi, Jean-Marc Panaud
Bactérie Helicobacter pylori en microscopie électronique à balayage. Agent causal de pathologies de l'estomac : elle est responsable des gastrites chroniques, d'ulcères gastriques et duodénaux et elle joue un rôle important dans la genèse des cancers gastriques (adénocarcinomes et lymphomes).
Publication : The Journal of biological chemistry

Deacetylases required for muramic-δ-lactam production are involved in sporulation, germination, and heat resistance

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in The Journal of biological chemistry - 28 Sep 2018

Coullon H, Rifflet A, Wheeler R, Janoir C, Boneca IG, Candela T

Link to Pubmed [PMID] – 30266804

J. Biol. Chem. 2018 11;293(47):18040-18054

Spores are produced by many organisms as a survival mechanism activated in response to several environmental stresses. Bacterial spores are multilayered structures, one of which is a peptidoglycan layer called the cortex, containing muramic-δ-lactams that are synthesized by at least two bacterial enzymes, the muramoyl-l-alanine amidase CwlD and the -deacetylase PdaA. This study focused on the spore cortex of , a Gram-positive, toxin-producing anaerobic bacterial pathogen that can colonize the human intestinal tract and is a leading cause of antibiotic-associated diarrhea. Using ultra-HPLC coupled with high-resolution MS, here we found that the spore cortex of the 630Δ strain differs from that of Among these differences, the muramic-δ-lactams represented only 24% in , compared with 50% in CD630_14300 and CD630_27190 were identified as genes encoding the -deacetylases PdaA1 and PdaA2, required for muramic-δ-lactam synthesis. In a mutant, only 0.4% of all muropeptides carried a muramic-δ-lactam modification, and muramic-δ-lactams were absent in the cortex of a double mutant. Of note, the mutant exhibited decreased sporulation, altered germination, decreased heat resistance, and delayed virulence in a hamster infection model. These results suggest a much greater role for muramic-δ-lactams in than in other bacteria, including In summary, the spore cortex of contains lower levels of muramic-δ-lactams than that of , and PdaA1 is the major -deacetylase for muramic-δ-lactam biosynthesis in , contributing to sporulation, heat resistance, and virulence.

https://www.ncbi.nlm.nih.gov/pubmed/30266804