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© Institut Pasteur
Cells infected for 24 hrs with C. Trachomatis. The cell nuclei are labelled in blue, the bacteria appear yellow, within the inclusion lumen. A bacterial protein secreted out the inclusion into the host cytoplasm id labelled in red.
Publication : Journal of molecular biology

Crystallization and preliminary X-ray diffraction study of an endoglucanase from Clostridium thermocellum

Team: Structural Microbiology
Member: Pedro Alzari
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Journal of molecular biology - 01 May 1986

Joliff G, Béguin P, Millet J, Aubert JP, Alzari P, Juy M, Poljak RJ

Link to Pubmed [PMID] – 3783675

J. Mol. Biol. 1986 May;189(1):249-50

Endoglucanase D, a cellulose degradation enzyme from Clostridium thermocellum has been cloned in Escherichia coli, purified and crystallized. The crystals are trigonal, space group P3(1)12 (or P3(2)12) with a = 57.7 (+/- 0.1) A, c = 192.1 (+/- 0.2) A, and diffract X-rays to a resolution of 2.8 A. They are suitable for a high-resolution X-ray diffraction analysis.