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© Research
Publication : Biochimie

Coprecipitation of topoisomerase activity with simian virus 40 nucleoprotein complexes by divalent cations

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Biochimie - 01 Jan 1980

Hamelin C, Yaniv M

Link to Pubmed [PMID] – 17941182

Biochimie 1980;62(4):261-5

Simian virus 40 (SV40) nucleoprotein complexes extracted from nuclei of infected monkey cells (CV1) were precipitated with Ca2+, Mg2+, and Mn2+ divalent cations. Most of the viral chromatin but only a fraction of the proteins in the crude nuclear extracts were recovered after precipitation with 10 mM MgCl2. At this optimal concentration, DNA topoisomerase activity (nicking closing enzyme) coprecipitated with the SV40 nucleoprotein complexes.

http://www.ncbi.nlm.nih.gov/pubmed/17941182