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© Research
Publication : Nucleic acids research

Nicking-closing enzyme is associated with SV40 DNA in vivo as a sodium dodecyl sulfate-resistant complex

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Nucleic acids research - 10 Oct 1979

Hamelin C, Yaniv M

Link to Pubmed [PMID] – 228247

Nucleic Acids Res. 1979 Oct;7(3):679-87

A fraction of the cellular nicking-closing (NC) enzyme cosediments with SV40 chromatin isolated after Triton X-100 treatment of infected cells nuclei. Extraction of viral DNA according to the Hirt procedure by treatment of infected cells with sodium dodecyl sulfate (SDS) followed by sedimentation in sucrose gradient to separate the DNA from the bulk of detergent also revealed NC activity associated with DNA. Reconstitution experiments showed that only prebinding of the NC enzyme to DNA protects it against irreversible inactivation by SDS. These results suggest that a fraction of the cellular NC activity is indeed associated with the viral chromosome in vivo.

http://www.ncbi.nlm.nih.gov/pubmed/228247