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© Research
Publication : European journal of biochemistry / FEBS

Conformational studies of d(m5CpGpm5CpG) and d(CpGpCpG) by 1H and 31P NMR

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in European journal of biochemistry / FEBS - 15 Dec 1986

Delepierre M, Langlois D'Estaintot B, Igolen J, Roques BP

Link to Pubmed [PMID] – 3024977

Eur. J. Biochem. 1986 Dec;161(3):571-7

Exhaustive conformational studies of d(CpG)2 and d(m5CpG)2, two convenient targets for DNA bisintercalating drugs, have been carried out by 1H and 31P NMR in low salt concentration and in the presence of 30% ethanol. Unambiguous 31P assignments of the B for are obtained with low-power heteronuclear decoupling experiments, while 31P assignments in the Z form are obtained by two-dimensional homonuclear chemical exchange experiments. The 31P chemical shifts and 3JH3’P coupling constants studied at various temperatures in methylated and non-methylated tetranucleotides, are interpreted as resulting from conformational differences between the compounds. These features are corroborated by homonuclear proton nuclear Overhauser effect experiments showing the steric role of the 5-methylcytosine in the induction of an alternating B form in d(m5CpG)2.

http://www.ncbi.nlm.nih.gov/pubmed/3024977