Link to HAL – pasteur-04331595
Link to DOI – 10.1007/978-1-0716-3445-5_4
Laure Journet; Eric Cascales. Bacterial Secretion Systems, 2715, Springer US, 2023, Methods in Molecular Biology, 978-1-0716-3444-8. ⟨10.1007/978-1-0716-3445-5_4⟩
Bacterial lipoproteins are characterized by fatty acids, derived from membrane phospholipids, which are covalently attached to their amino terminus via posttranslational modification in the cytoplasmic membrane. Here, I describe the detection of one of the intermediate forms of lipoprotein, diacylglyceryl-prolipoprotein, using 3H-palmitate labeling and inhibition of signal peptidase II (Lsp) by globomycin and detection by fluorography.