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© Research
Publication : FEMS microbiology letters

Comparison of protein patterns of Listeria monocytogenes grown in biofilm or in planktonic mode by proteomic analysis

Member: Abdelkader Namane
Member: Pascale Cossart
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in FEMS microbiology letters - 23 Apr 2002

Trémoulet F, Duché O, Namane A, Martinie B, Labadie JC,

Link to Pubmed [PMID] – 12023073

FEMS Microbiol. Lett. 2002 Apr;210(1):25-31

The proteome of a Listeria monocytogenes strain isolated from a food plant was investigated to study the differential protein pattern expressed by biofilms and planktonic bacteria. The approach used in this study was a combination of two-dimensional electrophoresis, matrix-assisted laser desorption ionization-time of flight and database searches for the protein identification. Thirty-one proteins varied significantly between the two growth conditions. Twenty-two and nine proteins were up- and down-regulated respectively and nine proteins were successfully identified. The variations of the protein patterns indicated that the biofilm development is probably controlled by specific regulation of protein expression involved at various levels of cellular physiology.