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© Research
Publication : Biochemical and biophysical research communications

Comparative modelling and immunochemical reactivity of Escherichia coli UMP kinase

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Biochemical and biophysical research communications - 31 May 2002

Labesse G, Bucurenci N, Douguet D, Sakamoto H, Landais S, Gagyi C, Gilles AM, Bârzu O

Link to Pubmed [PMID] – 12054759

Biochem. Biophys. Res. Commun. 2002 May;294(1):173-9

Bacterial UMP kinases do not exhibit any sequence homology with other nucleoside monophosphate kinases described so far, and appear under oligomeric forms, submitted to complex regulation by nucleotides. We propose here a structural model of UMP kinase from Escherichia coli based on the conservation of the fold of carbamate kinase whose crystal structure was recently solved. Despite sequence identity of only 18% over 203 amino acids, alignment of UMP kinase from E. coli with carbamate kinase from Enterococcus faecalis by hydrophobic cluster analysis and threading suggested the conservation of the overall structure, except for a small subdomain (absent in UMP kinase). The modelled dimer suggested conservation of the dimer interface observed in carbamate kinase while interaction of UMP kinase with a monoclonal antibody (Mab 44-2) suggests a three in-plane dimer subunit arrangement. The model was analyzed in light of various modified forms of UMP kinase obtained by site-directed mutagenesis.

http://www.ncbi.nlm.nih.gov/pubmed/12054759