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© Artur Scherf
Scanning Electron Microscopy of Red Blood Cell infected by Plasmodium falciparum.
Publication : Research in immunology

Cloning and expression of genomic DNA sequences coding for putative erythrocyte membrane-associated antigens of Plasmodium falciparum

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Research in immunology - 01 Mar 1991

Kun J, Hesselbach J, Schreiber M, Scherf A, Gysin J, Mattei D, Pereira da Silva L, Müller-Hill B

Link to Pubmed [PMID] – 1896607

Res. Immunol. 1991 Mar-Apr;142(3):199-210

Genomic DNA fragments of Plasmodium falciparum generated by mung bean nuclease digestion were cloned in the lambda expression vector lambda JK2. The resulting library was screened with a rabbit antiserum raised against purified membranes of P. falciparum-infected erythrocytes and with a serum pool from immune humans from an endemic area of Liberia. Positive clones were rescreened with a series of human and monkey sera. Twelve selected clones were analysed in detail. Four of them corresponded to already described membrane-associated P. falciparum antigens. The other positive clones contained inserts which, according to the nucleotide sequence, Southern blot analysis and immunological characteristics, correspond to so far unknown antigens.

http://www.ncbi.nlm.nih.gov/pubmed/1896607