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© Pierre Lafaye
Astrocytes marqués par des anticorps VHH anti-GFAP. Des anticorps d'alpagas dirigés contre une protéine spécifique des astrocytes, la GFAP (Glial Fibrillary Acidic Protein), ont été obtenus à partir de camélidés immunisés. La partie VHH (partie de l'anticorps qui reconnaît l'antigène) a été exprimée sous forme recombinante chez Escherichia coli.
Publication : Journal of virological methods

Cloning and expression of functional single-chain Fv antibodies directed against NIa and coat proteins of potato virus Y

Team: Antibody Engineering
Member: Pierre Lafaye
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Journal of virological methods - 01 Aug 2006

Rouis S, Lafaye P, Jaoua-Aydi L, Sghaier Z, Ayadi H, Gargouri-Bouzid R

Link to Pubmed [PMID] – 16884787

J. Virol. Methods 2006 Oct;137(1):1-6

Three single-chain variable fragment (scFv) antibodies recognizing the nuclear inclusion a (NIa) and capsid proteins of potato virus Y were obtained from two mouse derived hybridoma clones secreting, respectively, an anti-NIa (22-1) and an anti-coat protein (136-13) monoclonal antibodies. The first monoclonal antibody was able to inhibit in vitro the PVY polyprotein cleavage by blocking the NIa protease activity. The amplified scFv cDNAs were first inserted into the TOPO vector and then sequenced. Several recombinant E. coli clones carrying the accurate scFv sequences were selected and the corresponding cDNAs were subcloned in pHEN phagemid and transferred in E. coli strain. The expressed scFv fragments showed an antibody activity that recognized the viral target proteins in infected tissues. Their activity was comparable to the parental monoclonal antibodies.