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© Research
Publication : BMC biotechnology

Characterization of single chain antibody targets through yeast two hybrid

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in BMC biotechnology - 22 Aug 2010

Vielemeyer O, Nizak C, Jimenez AJ, Echard A, Goud B, Camonis J, Rain JC, Perez F

Link to Pubmed [PMID] – 20727208

BMC Biotechnol. 2010;10:59

Due to their unique ability to bind their targets with high fidelity, antibodies are used widely not only in biomedical research, but also in many clinical applications. Recombinant antibodies, including single chain variable fragments (scFv), are gaining momentum because they allow powerful in vitro selection and manipulation without loss of function. Regardless of the ultimate application or type of antibody used, precise understanding of the interaction between the antibody’s binding site and its specific target epitope(s) is of great importance. However, such data is frequently difficult to obtain.

http://www.ncbi.nlm.nih.gov/pubmed/20727208