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© Research
Publication : Scientific reports

Characterization of Klebsiella pneumoniae isolated from patients suspected of pulmonary or bubonic plague during the Madagascar epidemic in 2017.

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Scientific reports - 27 Apr 2022

Rakotondrasoa A, Andrianonimiadana LM, Rahajandraibe S, Razafimahatratra S, Andrianaivoarimanana V, Rahelinirina S, Crucitti T, Brisse S, Jeannoda V, Rajerison M, Collard JM

Link to Pubmed [PMID] – 35477743

Link to DOI – 10.1038/s41598-022-10799-4

Sci Rep 2022 Apr; 12(1): 6871

Klebsiella pneumoniae can lead to a wide range of diseases including pneumonia, bloodstream and urinary tract infections. During a short period of a pulmonary plague epidemic in October 2017 in Madagascar, 12 K. pneumoniae isolates were identified in ten sputum and two buboes aspirate samples. These isolates were from 12 patients suspected of plague, without epidemiological relationships, but were negative for Yersinia pestis in culture. Data were collected from the plague national surveillance system. The isolates were characterized by antimicrobial susceptibility testing and whole-genome sequencing. Real-time PCR was performed to confirm the presence of K. pneumoniae DNA in buboes. All isolates were identified as K. pneumoniae sensu stricto. Five isolates were extended-spectrum β-lactamases producers; eleven different sequence types were identified. Five isolates belonged to known hypervirulent sequence types. Our results demonstrate community-acquired pneumonia caused by K. pneumoniae isolates in patients suspected of plague stressing the importance of bed-side differential diagnosis.