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© Research
Publication : Journal of virology

Characterization of a transcriptional promoter of human papillomavirus 18 and modulation of its expression by simian virus 40 and adenovirus early antigens

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Journal of virology - 01 Jan 1987

Thierry F, Heard JM, Dartmann K, Yaniv M

Link to Pubmed [PMID] – 3023691

J. Virol. 1987 Jan;61(1):134-42

RNA present in cells derived from cervical carcinoma that contained human papillomavirus 18 genomes was initiated in the 1.053-kilobase BamHI fragment that covered the complete noncoding region of this virus. When cloned upstream of the chloramphenicol acetyltransferase gene, this viral fragment directed the expression of the bacterial enzyme only in the sense orientation. Initiation sites were mapped around the ATG of open reading frame E6. This promoter was active in some human and simian cell lines, and its expression was modulated positively by simian virus 40 large T antigen and negatively by adenovirus type 5 E1a antigen.

http://www.ncbi.nlm.nih.gov/pubmed/3023691