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© Research
Publication : Microbiology (Reading, England)

Characterization of a cell-wall acid phosphatase (PhoAp) in Aspergillus fumigatus

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Microbiology (Reading, England) - 01 Sep 2002

Bernard M, Mouyna I, Dubreucq G, Debeaupuis JP, Fontaine T, Vorgias C, Fuglsang C, Latgé JP

Link to Pubmed [PMID] – 12213928

Microbiology (Reading, Engl.) 2002 Sep;148(Pt 9):2819-29

In the filamentous fungus Aspergillus fumigatus, the vast majority of the cell-wall-associated proteins are secreted proteins that are in transit in the cell wall. These proteins can be solubilized by detergents and reducing agents. Incubation of a SDS/beta-mercaptoethanol-treated cell-wall extract with various recombinant enzymes that hydrolyse cell-wall polysaccharides resulted in the release of a unique protein in minute amounts only after incubation of the cell wall in the presence of 1,3-beta-glucanase. Sequence analysis and biochemical studies showed that this glycoprotein, with an apparent molecular mass of 80 kDa, was an acid phosphatase (PhoAp) that was active on both phosphate monoesters and phosphate diesters. PhoAp is a glycosylphosphatidylinositol-anchored protein that was recovered in the culture filtrate and cell-wall fraction of A. fumigatus after cleavage of its anchor. It is also a phosphate-repressible acid phosphatase. The absence of PhoAp from a phosphate-rich medium was not associated with a reduction in fungal growth, indicating that this cell-wall-associated protein does not play a role in the morphogenesis of A. fumigatus.