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© Artur Scherf
Scanning Electron Microscopy of Red Blood Cell infected by Plasmodium falciparum.
Publication : Nucleic acids research

Characterisation of P. falciparum antigenic determinants isolated from a genomic expression library by differential antibody screening

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Nucleic acids research - 11 Jun 1985

Langsley G, Scherf A, Mercereau-Puijalon O, Koenen M, Kahane B, Mattei D, Guillotte M, Sibilli L, Garner I, Müller-Hill B

Link to Pubmed [PMID] – 2409532

Nucleic Acids Res. 1985 Jun;13(11):4191-202

A genomic expression library of P.falciparum has been differentially screened with a number of immune sera. The response of 9 clones to the various sera is presented, together with the DNA sequence encoding the epitopes. All but one clone are extremely A+T rich and unlike the other P.falciparum epitopes described, are not composed of amino acid repeats. One clone, which responds specifically with a protective serum, has been analysed in detail. The epitope is carried on a 160kd antigen which is transcribed from a single gene to give a protein expressed in all of the erythrocytic forms. DNA sequence of this clone reveals it to have more than one open reading frame, only one of which is transcribed in the blood stages. The possible significance of the other open readings frames is discussed.

http://www.ncbi.nlm.nih.gov/pubmed/2409532