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© Research
Publication : Journal of proteomics

Cellular and proteomics analysis of the endomembrane system from the unicellular Entamoeba histolytica

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Journal of proteomics - 07 Aug 2014

Perdomo D, Aït-Ammar N, Syan S, Sachse M, Jhingan GD, Guillén N

Link to Pubmed [PMID] – 25109464

J Proteomics 2015 Jan;112:125-40

UNLABELLED: Entamoeba histolytica is the protozoan parasite agent of amoebiasis, an infectious disease of the human intestine and liver. Specific active pathogenic factors are secreted toward the external milieu upon interaction of the parasite with human tissue. Trafficking dynamics and secretion of these factors is not known and characterization of the dynamics interplay of subcellular compartments such as the ER or Golgi apparatus is still pending. In this work, we took advantage of cell fractionation and a wide proteomic analysis to search for principal components of the endomembrane system in E. histolytica. Over 1500 proteins were identified and the two top categories contained components of trafficking machinery and GTPases. Trafficking related proteins account for over 100 markers from the ER, Golgi, MVB, and retromers. The lack of important components supporting Golgi polarization was also highlighted. The data further describe principal components of the endosomal traffic highlighting Alix in isolated vesicles and during parasite division.

BIOLOGICAL SIGNIFICANCE: This work represents the first in-depth proteomics analysis of subcellular compartments in E. histolytica and allows a detailed map of vesicle traffic components in an ancient single-cell organism that lacks a stereotypical ER and Golgi apparatus to be established.