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© Research
Publication : The Journal of biological chemistry

Bordetella pertussis adenylate cyclase toxin. Structural and functional independence of the catalytic and hemolytic activities

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in The Journal of biological chemistry - 01 Jul 1992

Sakamoto H, Bellalou J, Sebo P, Ladant D

Link to Pubmed [PMID] – 1618862

J. Biol. Chem. 1992 Jul;267(19):13598-602

The Bordetella pertussis calmodulin-dependent adenylate cyclase (CyaA) is a 1706-residue-long toxin, endowed with hemolytic activity. We have constructed B. pertussis mutant strains producing modified CyaAs devoid of adenylate cyclase activity. Our results show that such modified CyaAs display hemolytic activity identical to the wild-type toxin, thus demonstrating that the hemolytic activity is independent of the adenylate cyclase activity. Furthermore, B. pertussis and Escherichia coli strains producing CyaA lacking the catalytic domain (residues 1-373) were constructed. The truncated protein exhibits hemolytic activity comparable to the wild-type toxin, thus establishing that the carboxyl-terminal 1332 residues alone are endowed with hemolytic activity. Together, these findings show that adenylate cyclase and hemolytic activities are located in two distinct regions of the molecule (respectively, approximately amino acids 1-400 and 401-1706) and that the two regions of CyaA are functionally independent.

http://www.ncbi.nlm.nih.gov/pubmed/1618862