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© Research
Publication : The Journal of biological chemistry

Bordetella pertussis adenylate cyclase. Purification, characterization, and radioimmunoassay

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in The Journal of biological chemistry - 01 Dec 1986

Ladant D, Brezin C, Alonso JM, Crenon I, Guiso N

Link to Pubmed [PMID] – 2877986

J. Biol. Chem. 1986 Dec;261(34):16264-9

The extracellular adenylate cyclase of Bordetella pertussis was purified either as a free enzyme or as a complex with calmodulin. The purified enzyme has a specific activity of 1600 mumol of cAMP min-1 X mg-1 and exists under two molecular forms of 45 and 43 kDa which are apparently structurally related. Calmodulin increased considerably the resistance of adenylate cyclase to inactivation by trypsin. Although trypsin cleaved the adenylate cyclase-calmodulin complex, the digested fragments remained associated by noncovalent interactions in an active conformation. Specific mouse anti-adenylate cyclase antibodies inhibit adenylate cyclase activity and were used to develop a specific radioimmunoassay that allows detection of as little as 5 ng of adenylate cyclase in culture supernatants.

http://www.ncbi.nlm.nih.gov/pubmed/2877986