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© Research
Publication : Applied optics

Blind deconvolution for thin-layered confocal imaging

Team: Biological Image Analysis
Member: Jean-Christophe Olivo-Marin
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Applied optics - 01 Aug 2009

Pankajakshan P, Zhang B, Blanc-Féraud L, Kam Z, Olivo-Marin JC, Zerubia J

Link to Pubmed [PMID] – 19649049

Appl Opt 2009 Aug;48(22):4437-48

We propose an alternate minimization algorithm for estimating the point-spread function (PSF) of a confocal laser scanning microscope and the specimen fluorescence distribution. A three-dimensional separable Gaussian model is used to restrict the PSF solution space and a constraint on the specimen is used so as to favor the stabilization and convergence of the algorithm. The results obtained from the simulation show that the PSF can be estimated to a high degree of accuracy, and those on real data show better deconvolution as compared to a full theoretical PSF model.