Search anything and hit enter
  • Teams
  • Members
  • Projects
  • Events
  • Calls
  • Jobs
  • publications
  • Software
  • Tools
  • Network
  • Equipment

A little guide for advanced search:

  • Tip 1. You can use quotes "" to search for an exact expression.
    Example: "cell division"
  • Tip 2. You can use + symbol to restrict results containing all words.
    Example: +cell +stem
  • Tip 3. You can use + and - symbols to force inclusion or exclusion of specific words.
    Example: +cell -stem
e.g. searching for members in projects tagged cancer
Search for
Count
IN
OUT
Content 1
  • member
  • team
  • department
  • center
  • program_project
  • nrc
  • whocc
  • project
  • software
  • tool
  • patent
  • Administrative Staff
  • Assistant Professor
  • Associate Professor
  • Clinical Research Assistant
  • Clinical Research Nurse
  • Clinician Researcher
  • Department Manager
  • Dual-education Student
  • Full Professor
  • Honorary Professor
  • Lab assistant
  • Master Student
  • Non-permanent Researcher
  • Nursing Staff
  • Permanent Researcher
  • Pharmacist
  • PhD Student
  • Physician
  • Post-doc
  • Prize
  • Project Manager
  • Research Associate
  • Research Engineer
  • Retired scientist
  • Technician
  • Undergraduate Student
  • Veterinary
  • Visiting Scientist
  • Deputy Director of Center
  • Deputy Director of Department
  • Deputy Director of National Reference Center
  • Deputy Head of Facility
  • Director of Center
  • Director of Department
  • Director of Institute
  • Director of National Reference Center
  • Group Leader
  • Head of Facility
  • Head of Operations
  • Head of Structure
  • Honorary President of the Departement
  • Labex Coordinator
Content 2
  • member
  • team
  • department
  • center
  • program_project
  • nrc
  • whocc
  • project
  • software
  • tool
  • patent
  • Administrative Staff
  • Assistant Professor
  • Associate Professor
  • Clinical Research Assistant
  • Clinical Research Nurse
  • Clinician Researcher
  • Department Manager
  • Dual-education Student
  • Full Professor
  • Honorary Professor
  • Lab assistant
  • Master Student
  • Non-permanent Researcher
  • Nursing Staff
  • Permanent Researcher
  • Pharmacist
  • PhD Student
  • Physician
  • Post-doc
  • Prize
  • Project Manager
  • Research Associate
  • Research Engineer
  • Retired scientist
  • Technician
  • Undergraduate Student
  • Veterinary
  • Visiting Scientist
  • Deputy Director of Center
  • Deputy Director of Department
  • Deputy Director of National Reference Center
  • Deputy Head of Facility
  • Director of Center
  • Director of Department
  • Director of Institute
  • Director of National Reference Center
  • Group Leader
  • Head of Facility
  • Head of Operations
  • Head of Structure
  • Honorary President of the Departement
  • Labex Coordinator
Search
Go back
Scroll to top
Share
© Research
Publication : European journal of immunology

Biochemical characterization and biological effects of partially purified B cell-activating factor (BCAF)

Team: Ecology and Emergence of Arthropod-borne Pathogens
Member: Claude Roth
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in European journal of immunology - 01 Apr 1988

Roth C, Moreau JL, Korner M, Jankovic D, Thèze J

Link to Pubmed [PMID] – 3259186

Eur. J. Immunol. 1988 Apr;18(4):577-84

B cell-activating factor (BCAF) has been characterized and partially purified from the supernatant of the murine tumor T cell line 373. BCAF has an apparent molecular mass of 15 to 20 kDa when analyzed by Superose 12 fast protein liquid chromatography (FPLC) gel filtration and a pI of 4.5 to 5.0 when analyzed by FPLC chromatofocusing. Concentrated supernatant was applied to a nickel chelating column and unadsorbed active material was further purified by two sequential C4 reverse-phase high-performance liquid chromatography steps. This purification procedure allowed a complete separation of interleukin 2 and interleukin 4 activities from BCAF. This partially purified BCAF induces Ia expression, cell size increase and proliferation of small resting B cells. Furthermore, we have shown that the activity of partially purified BCAF is insensitive to treatment by monoclonal antibodies specific for interleukin 4 and interleukin 5.