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© Thierry Blisnick & Philippe Bastin, Institut Pasteur
Bloodstream Trypanosoma brucei cell
Publication : The EMBO journal

Binding of IFT22 to the intraflagellar transport complex is essential for flagellum assembly

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in The EMBO journal - 02 Apr 2019

Wachter S, Jung J, Shafiq S, Basquin J, Fort C, Bastin P, Lorentzen E

Link to Pubmed [PMID] – 30940671

EMBO J. 2019 Apr;

Intraflagellar transport (IFT) relies on motor proteins and the IFT complex to construct cilia and flagella. The IFT complex subunit IFT22/RabL5 has sequence similarity with small GTPases although the nucleotide specificity is unclear because of non-conserved G4/G5 motifs. We show that IFT22 specifically associates with G-nucleotides and present crystal structures of IFT22 in complex with GDP, GTP, and with IFT74/81. Our structural analysis unravels an unusual GTP/GDP-binding mode of IFT22 bypassing the classical G4 motif. The GTPase switch regions of IFT22 become ordered upon complex formation with IFT74/81 and mediate most of the IFT22-74/81 interactions. Structure-based mutagenesis reveals that association of IFT22 with the IFT complex is essential for flagellum construction in although IFT22 GTP-loading is not strictly required.

https://www.ncbi.nlm.nih.gov/pubmed/30940671