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© Research
Publication : Analytical biochemistry

Analysis of pyruvic acid acetal containing polysaccharides by methanolysis and reductive cleavage methods

Team: Archived: Aspergillus
Member: Thierry Fontaine
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Analytical biochemistry - 01 Dec 1991

Fontaine T, Talmont F, Dutton GG, Fournet B

Link to Pubmed [PMID] – 1812780

Anal. Biochem. 1991 Dec;199(2):154-61

The mass spectra of permethylated methyl 4,6-O-(1-carbomethoxyethylidene)-D-hexopyranoside and 1,5-anhydro-D-hexitol of glucose, galactose, and mannose and permethylated methyl 5,6-O-(1-carbomethoxyethylidene)-D-galactofuranoside and 1,4-anhydro-D-galactitol have been determined. The stability of each compound toward methanolysis and reductive cleavage is discussed. These techniques permit the identification of the acetalic linkages of pyruvic acid present in polysaccharides.